Construction of co‐expression modules related to survival by WGCNA and identification of potential prognostic biomarkers in glioblastoma

Glioblastoma (GBM) is a malignant brain tumour with poor prognosis. The potential pathogenesis and therapeutic target are still need to be explored. Herein, TCGA expression profile data clinical information were downloaded, the WGCNA was conducted. Hub genes which closely related prognosis of GBM obtained. Further, relationship between interest GBM, immune microenvironment analysed. Patients from divided into high- low-risk group. applied group black module lowest preservation identified could distinguish level these two groups. top 10 hub patients GO analysis showed biological process mainly enriched in: Cell cycle, Progesterone-mediated oocyte maturation Oocyte meiosis. CDCA5 CDCA8 screened out as interest. We found that their levels overall survival. difference resulted database proved both highly expressed in GBM. After transfection U87-MG cells small interfering RNA, it revealed knockdown influence behaviours proliferation, clonogenicity apoptosis cells. Then, single-gene performed. had good correlations regulate cell cycle p53 signalling pathway. Moreover, high amplification correlated CD8+ T while CD4+ These results might provide new molecular targets intervention strategy for